產(chǎn)品名稱:假單胞菌瓊脂基礎(chǔ)培養(yǎng)基(CN瓊脂)
Product name:CN Agar
產(chǎn)品用途:用于假單胞菌的選擇性分離培養(yǎng)。
Product usage: For selective isolation and culture of pseudomonas.
檢驗(yàn)原理
Test principle
明膠蛋白胨和酸水解酪蛋白提供氮源;甘油提供碳源;硫酸鉀和氯化鎂可促進(jìn)綠膿色素的產(chǎn)生;瓊脂是培養(yǎng)基的凝固劑;萘啶酮酸抑制非假單胞菌的革蘭氏陰性桿菌。
Gelatin peptone and acid hydrolyzed casein provide nitrogen sources. Glycerol provides carbon source; Potassium sulfate and magnesium chloride can promote the production of pyocyanin. AGAR is the coagulant of medium; Nalidixonic acid inhibits Gram-negative bacilli that are not pseudomonas.
配方成分
Formula ingredients
配方 Formula |
含量(g/L) Content(g/L) |
明膠蛋白胨 Gelatin peptone |
16.0 |
胰蛋白胨 Tryptone |
10.0 |
硫酸鉀 Potassium sulfate |
10.0 |
氯化鎂 Magnesium chloride |
1.4 |
十六烷基三甲溴化銨Cetyltrimethylammonium bromide |
0.2 |
萘啶酮酸 Nalidixonic acid |
0.015 |
瓊脂 Agar |
15.0 |
pH |
7.1±0.2 25℃ |
使用方法
Usage
秤取本品52.6g,加入蒸餾水1000ml,另加入10ml甘油,攪拌加熱煮沸至完全溶解,分裝,121℃高壓滅菌15分鐘,備用。
注:甘油需用戶自備。
注:培養(yǎng)基中含少量氯化鎂,滅菌后可能出現(xiàn)微量沉淀。
Weigh 52.6g of this product, add 1000ml of distilled water, add another 10ml of glycerol, stirring heating and boiling until completely dissolved, divided, 121 ℃ autoclave sterilization for 15 minutes, standby.
Note: Glycerol should be prepared by the user.
Note: The medium contains a small amount of magnesium chloride, trace precipitation may occur after sterilization.
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